9BIF
Fab B11-OspCA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 96.737, 97.294, 168.343 |
| Unit cell angles | 97.60, 90.27, 106.21 |
Refinement procedure
| Resolution | 48.620 - 3.090 |
| R-factor | 0.2425 |
| Rwork | 0.240 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.968 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Number of reflections | 96279 | 9069 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 90.8 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.750 | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100 mM sodium HEPES [pH 6.5], 18.6% PEG 6K, and 6.6 mM prasseodymium acetate |
