Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.19499 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.683, 101.920, 66.731 |
| Unit cell angles | 90.00, 90.81, 90.00 |
Refinement procedure
| Resolution | 39.060 - 1.600 |
| R-factor | 0.1671 |
| Rwork | 0.165 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.913 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.060 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.068 | 0.683 |
| Rpim | 0.045 | 0.615 |
| Number of reflections | 76408 | 2034 |
| <I/σ(I)> | 10.8 | 1.4 |
| Completeness [%] | 91.1 | 49.3 |
| Redundancy | 3.3 | |
| CC(1/2) | 0.992 | 0.470 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 28% PEG 3350, 0.1M Bis Tris, pH 5.5, 150 mM NaCl |
