9BF2
MID domain of Ago2 bound to UMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | LIQUID ANODE |
Source details | Excillum MetalJet D2+ 70 kV |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-06 |
Detector | Bruker PHOTON III |
Wavelength(s) | 1.3418 |
Spacegroup name | P 1 |
Unit cell lengths | 39.608, 46.339, 64.920 |
Unit cell angles | 87.58, 74.68, 85.87 |
Refinement procedure
Resolution | 21.580 - 1.590 |
R-factor | 0.1645 |
Rwork | 0.163 |
R-free | 0.18690 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.288 |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.580 | 1.652 |
High resolution limit [Å] | 1.590 | 1.590 |
Number of reflections | 118253 | 11241 |
<I/σ(I)> | 15.2 | 1.98 |
Completeness [%] | 99.2 | |
Redundancy | 7.2 | |
CC(1/2) | 0.999 | 0.587 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | Drop was 1:1 15 mg/ml MID-UMP and 1.4M sodium citrate tribasic dihydrate, 0.1M HEPES pH7.5 |