9BF2

MID domain of Ago2 bound to UMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	Excillum MetalJet D2+ 70 kV
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-06
Detector	Bruker PHOTON III
Wavelength(s)	1.3418
Spacegroup name	P 1
Unit cell lengths	39.608, 46.339, 64.920
Unit cell angles	87.58, 74.68, 85.87

Refinement procedure

Resolution	21.580 - 1.590
R-factor	0.1645
Rwork	0.163
R-free	0.18690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.288
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	MOLREP
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.580	1.652
High resolution limit [Å]	1.590	1.590
Number of reflections	118253	11241
<I/σ(I)>	15.2	1.98
Completeness [%]	99.2
Redundancy	7.2
CC(1/2)	0.999	0.587

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	Drop was 1:1 15 mg/ml MID-UMP and 1.4M sodium citrate tribasic dihydrate, 0.1M HEPES pH7.5