9BEV

Structure of GH110A in complex with a lambda-carrageenan oligosaccharide

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-11
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.541
Spacegroup name	C 1 2 1
Unit cell lengths	231.513, 77.478, 116.039
Unit cell angles	90.00, 113.21, 90.00

Refinement procedure

Resolution	28.810 - 2.590
R-factor	0.2381
Rwork	0.236
R-free	0.27190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.503
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.589	2.600
Rmerge	0.194	0.535
Rpim	0.089	0.351
Number of reflections	58719	2903
<I/σ(I)>	7.3
Completeness [%]	99.8
Redundancy	7.3
CC(1/2)	0.975	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	40% pentaerythritol propoxylate, 0.05M KCl, 0.05M HEPES (pH 7.5)