9BEL

Tungstate binding protein (Tungbindin) from Eubacterium limosum with five Tungstates bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-28
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	74.306, 74.306, 148.437
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.880 - 2.680
R-factor	0.209
Rwork	0.203
R-free	0.26530
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.250
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	AutoSol
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.880	2.730
High resolution limit [Å]	2.680	2.683
Rmerge	0.173	1.018
Rmeas	0.177	1.049
Rpim	0.036	0.248
Number of reflections	21946	724
<I/σ(I)>	31.75
Completeness [%]	98.8
Redundancy	22.9
CC(1/2)		0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium citrate tribasic pH 7.0 20% w/v Polyethylene glycol 3,350