9BEH
Structure of GH110A in complex with galactose-6-sulfate
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-05 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.541 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 230.112, 77.477, 116.031 |
| Unit cell angles | 90.00, 113.39, 90.00 |
Refinement procedure
| Resolution | 19.969 - 2.247 |
| R-factor | 0.2028 |
| Rwork | 0.201 |
| R-free | 0.23240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.904 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.290 |
| High resolution limit [Å] | 2.247 | 2.247 |
| Rmerge | 0.110 | 0.566 |
| Rpim | 0.065 | 0.373 |
| Number of reflections | 86709 | 4700 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.992 | 0.815 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 25% pentaerythritol propoxylate, 0.05M KCl, 0.05M HEPES (pH 7.5) |
