9BEB
Tungstate binding protein (Tungbindin) from Eubacterium limosum with eight Tungstates bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-21 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 74.816, 74.816, 156.312 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.080 - 1.850 |
| R-factor | 0.1622 |
| Rwork | 0.161 |
| R-free | 0.18740 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.677 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (1.21) |
| Refinement software | PHENIX (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.080 | 1.880 |
| High resolution limit [Å] | 1.846 | 1.846 |
| Rmerge | 0.081 | 0.207 |
| Rmeas | 0.092 | 0.297 |
| Rpim | 0.149 | |
| Number of reflections | 67407 | 1258 |
| <I/σ(I)> | 42.92 | 3.68 |
| Completeness [%] | 92.5 | 66.1 |
| Redundancy | 18.3 | 3.4 |
| CC(1/2) | 0.945 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Magnesium acetate, 0.1 M Sodium cacodylate pH 6.5, 15%(w/v) PEG 6000 |
