9BDZ
TA-centric NF-kappaB RelA binding DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-11-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.078, 46.233, 98.646 |
| Unit cell angles | 89.90, 89.86, 60.10 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.26197 |
| Rwork | 0.258 |
| R-free | 0.33907 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.818 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.110 | 0.625 |
| Number of reflections | 12092 | 1100 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 88.9 | 94.4 |
| Redundancy | 2.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG3350 8%, Sodium Acetate pH 3.5 100 mM, 50 mM Zinc Acetate |
