9BDV
NF-kappaB RelA homo-dimer bound to TA-centric kappaB DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-11-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 118.654, 132.682, 45.663 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.660 - 1.900 |
| R-factor | 0.19302 |
| Rwork | 0.191 |
| R-free | 0.23048 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ram |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.961 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.060 | 1.900 |
| Rmerge | 0.994 | 0.044 | 2.558 |
| Total number of observations | 448525 | ||
| Number of reflections | 57610 | 3121 | 2961 |
| <I/σ(I)> | 8.5 | ||
| Completeness [%] | 99.6 | 94.4 | 99.9 |
| Redundancy | 7.8 | 6.9 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG3350, MES, Spermine, beta-octylglucoside |
