9BD5
Laccase from Bacillus licheniformis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER X8 PROTEUM |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2023-10-17 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 65 |
| Unit cell lengths | 94.800, 94.800, 271.658 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.700 - 2.700 |
| R-factor | 0.1915 |
| Rwork | 0.189 |
| R-free | 0.23220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.514 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5134) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.200 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.350 | |
| Number of reflections | 36830 | 3808 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 97.7 | |
| Redundancy | 3.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2 M ammonium sulfate as the reservoir and the drop was composed of a 3:1 ratio of protein (5 mg mL-1): reservoir solution |
