9BCM
Crystal structure of the glycosyltransferase UGT95A1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-04-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99988 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 62.518, 92.221, 92.643 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.110 - 2.360 |
R-factor | 0.2078 |
Rwork | 0.205 |
R-free | 0.23650 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.673 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.110 | 2.420 |
High resolution limit [Å] | 2.360 | 2.360 |
Number of reflections | 49224 | 2108 |
<I/σ(I)> | 4.27 | |
Completeness [%] | 99.9 | |
Redundancy | 6.1 | |
CC(1/2) | 0.974 | 0.002 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium acetate, 0.1 M MES pH 5.5 25% polyethylene glycol 3350 |