9BCF
Chimeric protein of crocodile allergen Cro p 1.0101 and GFP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 158.778, 158.778, 123.498 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.880 - 3.200 |
| R-factor | 0.20078 |
| Rwork | 0.199 |
| R-free | 0.23820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.260 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.071 | 1.213 |
| Rmeas | 0.131 | 1.460 |
| Rpim | 0.033 | 0.367 |
| Number of reflections | 26890 | 1321 |
| <I/σ(I)> | 17.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 15.2 | |
| CC(1/2) | 0.998 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M calcium acetate, 0.1 M sodium cacodylate pH 6.5, 40% v/v PEG 300 |
