9BCE
Shewanella oneidensis LysR family regulator SO0839 regulatory domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-08-28 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.040, 42.962, 112.092 |
| Unit cell angles | 90.00, 104.15, 90.00 |
Refinement procedure
| Resolution | 39.010 - 1.400 |
| R-factor | 0.1706 |
| Rwork | 0.170 |
| R-free | 0.19580 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.026 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.452 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.101 | |
| Rpim | 0.170 | |
| Number of reflections | 134465 | |
| <I/σ(I)> | 12.41 | |
| Completeness [%] | 94.8 | |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 27.5% v/v PEG-4000 100 mM HEPES/ Sodium hydroxide, pH 7.0 |
