Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.448, 71.416, 77.473 |
| Unit cell angles | 90.81, 107.19, 94.43 |
Refinement procedure
| Resolution | 39.110 - 2.520 |
| R-factor | 0.20807 |
| Rwork | 0.205 |
| R-free | 0.26452 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.940 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.110 | 2.660 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.099 | 0.416 |
| Rmeas | 0.135 | 0.567 |
| Rpim | 0.092 | 0.383 |
| Total number of observations | 8421 | |
| Number of reflections | 26716 | 3835 |
| <I/σ(I)> | 7.3 | 2.2 |
| Completeness [%] | 83.5 | |
| Redundancy | 2.2 | 2.2 |
| CC(1/2) | 0.986 | 0.708 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 10% PEG 4K, 10% 2-propanol, 0.1 M HEPES (pH 7.0) |
