9B9S
Crystal structure of the ligand binding domain of the Halomonas titanicae chemoreceptor Htc10 in complex with guanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-31 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.967697 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 178.537, 178.537, 89.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.540 - 3.600 |
| R-factor | 0.2544 |
| Rwork | 0.253 |
| R-free | 0.28620 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.562 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.380 | 3.940 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.124 | 0.836 |
| Rmeas | 0.132 | 0.893 |
| Rpim | 0.046 | 0.307 |
| Number of reflections | 6446 | 1530 |
| <I/σ(I)> | 8.2 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.1 | 8.4 |
| CC(1/2) | 0.998 | 0.828 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | sodium acetate pH 4.6, 2 M sodium formate |
