9B9M
Crystal structure of iron-bound FlcD from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9201 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.941, 114.061, 103.952 |
| Unit cell angles | 90.00, 102.54, 90.00 |
Refinement procedure
| Resolution | 29.350 - 2.070 |
| R-factor | 0.2003 |
| Rwork | 0.200 |
| R-free | 0.24110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.270 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.350 | 2.144 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.052 | 0.451 |
| Rmeas | 0.074 | 0.638 |
| Rpim | 0.052 | 0.451 |
| Number of reflections | 99235 | 9909 |
| <I/σ(I)> | 9.7 | 1.64 |
| Completeness [%] | 99.2 | 99.97 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.997 | 0.676 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES pH 7.5, 0.2 M MgCl2, 25% PEG 3350 |
