9B76
Crystal structure of humanized 44H10 Fab Version 21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03317 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.701, 86.068, 67.447 |
| Unit cell angles | 90.00, 105.73, 90.00 |
Refinement procedure
| Resolution | 35.870 - 1.730 |
| R-factor | 0.1771 |
| Rwork | 0.175 |
| R-free | 0.21170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.760 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Number of reflections | 41800 | 2076 |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 99.2 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.996 | 0.583 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 20% PEG3350, 0.2 M potassium iodide |
