9B72

Rec2 Domain from G. stearothermophilus Cas9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920105
Spacegroup name	C 1 2 1
Unit cell lengths	87.938, 72.353, 50.807
Unit cell angles	90.00, 122.72, 90.00

Refinement procedure

Resolution	42.750 - 1.490
R-factor	0.1718
Rwork	0.171
R-free	0.19660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.761
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.750	1.520
High resolution limit [Å]	1.490	1.490
Number of reflections	43163	2065
<I/σ(I)>	11.2
Completeness [%]	98.9
Redundancy	7.1
CC(1/2)	0.986	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.15 M calcium chloride, 15 % polyethylene glycol 6000, 0.1 M HEPES at pH 7.0