9B4S

Crystal structure of the RRAS2-p110alpha complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-29
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.97918
Spacegroup name	P 42 21 2
Unit cell lengths	181.488, 181.488, 96.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.260 - 3.100
R-factor	0.2322
Rwork	0.230
R-free	0.27170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.385
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.260	49.260	3.290
High resolution limit [Å]	3.100	9.160	3.100
Rmerge	0.067	0.028	2.038
Rmeas	0.071	0.030	2.154
Number of reflections	28076	1158	4491
<I/σ(I)>	19.12
Completeness [%]	94.8
Redundancy	9.3
CC(1/2)	0.999	0.999	0.435

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M (Bicine/Tris), 0.06 M Divalent mix, 30% (40%v/v PEG MME 500, 20%w/v PEG 20000)