9B49
K459Q variant of P. indica phosphate transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2019-05-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.116 |
| Spacegroup name | H 3 |
| Unit cell lengths | 173.670, 173.670, 172.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.860 - 3.100 |
| R-factor | 0.2377 |
| Rwork | 0.215 |
| R-free | 0.24040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.597 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.131 | 2.640 |
| Rmeas | 0.160 | 3.200 |
| Rpim | 0.092 | 1.860 |
| Number of reflections | 35004 | 3528 |
| <I/σ(I)> | 6.21 | 0.41 |
| Completeness [%] | 95.9 | 67.6 |
| Redundancy | 2.9 | 2.8 |
| CC(1/2) | 0.997 | 0.089 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 27% (W/V) pentaerythritol propoxylate, 5% polyethylene glycol 400,.2M KCl, .1M sodium citrate pH 5.5 |
