9B3G

Human Notch-1 EGFs 21-23

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-07-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.92
Spacegroup name	P 41
Unit cell lengths	41.920, 41.920, 61.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.920 - 1.550
R-factor	0.1961
Rwork	0.195
R-free	0.21830
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.684
Data reduction software	xia2
Data scaling software	xia2
Phasing software	AutoSol (1.18.2_3874)
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.920	1.590
High resolution limit [Å]	1.550	1.550
Rmerge	0.022	0.497
Number of reflections	15359	1109
<I/σ(I)>	27.1	2.5
Completeness [%]	99.7
Redundancy	4.7
CC(1/2)	0.999	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M Ammonium acetate 0.1 M BIS-Tris pH 5.5 25% (w/v) PEG3350