9AZT

CH67 Fab bound to A/Massachusetts/1/1990 influenza hemagglutinin head with a G189E mutation (1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	80.752, 91.700, 110.102
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.200 - 2.940
R-factor	0.2986
Rwork	0.294
R-free	0.33840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.755
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.200	3.120
High resolution limit [Å]	2.940	2.940
Number of reflections	17764	2695
<I/σ(I)>	22.92
Completeness [%]	99.2
Redundancy	6.6
CC(1/2)	1.000	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	10% PEG 8K, 100 mM HEPES, pH 7, 500 mM NaCl