9AZB

Crystal structure of LolTv5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-01
Detector	DECTRIS EIGER2 R 1M
Wavelength(s)	0.97946
Spacegroup name	P 32 2 1
Unit cell lengths	77.530, 77.530, 150.685
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.540 - 2.900
R-factor	0.287
Rwork	0.284
R-free	0.34230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.870
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.540	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.178	1.545
Rpim	0.044	0.391
Number of reflections	12161	6103
<I/σ(I)>	29.5
Completeness [%]	99.8	100
Redundancy	17.5	16.1
CC(1/2)	0.990	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M MES buffer, pH=5.8, 18%PEG4000