9AZA
Crystal structure of LolTv4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-05 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 74.239, 74.239, 154.669 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.120 - 2.840 |
| R-factor | 0.2285 |
| Rwork | 0.227 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.877 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.120 | 2.900 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rpim | 0.020 | 0.302 |
| Number of reflections | 11705 | 566 |
| <I/σ(I)> | 52 | |
| Completeness [%] | 96.2 | |
| Redundancy | 18.8 | |
| CC(1/2) | 0.997 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M MES buffer, pH=5.6, 18%PEG4000 |
