9AYD

Mitochondrial fission 1 (Fis1) protein structure Y38E mutation 1.53A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 2
Unit cell lengths	68.500, 81.440, 47.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.860 - 1.530
R-factor	0.1832
Rwork	0.181
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.137
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.861	38.861	1.570
High resolution limit [Å]	1.530	6.840	1.530
Rmerge	0.060	0.020	2.375
Rmeas	0.063	0.021	2.467
Number of reflections	40271	517	2961
<I/σ(I)>	22.25
Completeness [%]	99.2
Redundancy	12.8
CC(1/2)	1.000	0.999	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	292	0.1M Sodium Acetate (pH 4.5), 30% PEG smear low