9AXW

Nanobody NbJRI bound to yeast Pdc1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	101.449, 113.048, 155.496
Unit cell angles	79.42, 78.06, 67.02

Refinement procedure

Resolution	29.590 - 3.300
R-factor	0.1994
Rwork	0.196
R-free	0.23930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.533
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	BALBES
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.600	3.470
High resolution limit [Å]	3.300	3.300
Number of reflections	90822	12334
<I/σ(I)>	9.2
Completeness [%]	97.4
Redundancy	3.6
CC(1/2)	0.997	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	Bis-Tris propane 0.1 M pH 6.0, 0.2 M Sodium citrate, 22% w/v PEG3350, 5 mM TPP and 5 mM MgCl2