9AX6

Tricomplex of RMC-6236, KRAS G12D, and CypA

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	66.240, 84.140, 127.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.050 - 1.650
R-factor	0.1867
Rwork	0.185
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.050	1.709
High resolution limit [Å]	1.650	1.650
Rmerge	0.066	2.286
Rmeas	0.072	2.470
Rpim	0.028	0.928
Number of reflections	85162	8346
<I/σ(I)>	14.21	0.87
Completeness [%]	98.4
Redundancy	6.7
CC(1/2)	0.999	0.469

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Tris, pH 8.0, 25% PEG4000