9AW8

Crystal structure of trypsin at 150 Kelvin with benzamidine

Experimental procedure

Experimental method	LAUE
Source type	ROTATING ANODE
Source details	BRUKER TURBO X-RAY SOURCE
Temperature [K]	150
Detector technology	CMOS
Collection date	2023-11-03
Detector	BRUKER PHOTON 100
Wavelength(s)	1.54184
Spacegroup name	P 21 21 21
Unit cell lengths	53.658, 57.150, 64.795
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.751 - 1.496
Rwork	0.177
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.618
Data reduction software	SAINT
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.751	22.750
High resolution limit [Å]	1.496	1.500
Number of reflections	32548	1590
<I/σ(I)>	15.54
Completeness [%]	99.2
Redundancy	10
CC(1/2)	0.877	0.877

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M Potassium phosphate dibasic, 20% w/v Polyethylene glycol 3,350.