Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.458 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 134.620, 299.912, 144.663 |
| Unit cell angles | 90.00, 112.60, 90.00 |
Refinement procedure
| Resolution | 30.040 - 2.910 |
| R-factor | 0.1962 |
| Rwork | 0.194 |
| R-free | 0.23190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.549 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.040 | 2.960 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Rmerge | 0.267 | 1.526 |
| Rmeas | 0.289 | 1.647 |
| Rpim | 0.109 | 0.617 |
| Total number of observations | 1591363 | 79070 |
| Number of reflections | 228968 | 11282 |
| <I/σ(I)> | 7.4 | 1.5 |
| Completeness [%] | 99.2 | |
| Redundancy | 7 | 7 |
| CC(1/2) | 0.985 | 0.689 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM |
