Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-04 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.458 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 134.892, 299.287, 144.209 |
| Unit cell angles | 90.00, 112.78, 90.00 |
Refinement procedure
| Resolution | 45.740 - 3.440 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.25170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.490 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | DIMPLE |
| Refinement software | PHENIX (1.21.1.5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 3.500 |
| High resolution limit [Å] | 3.440 | 3.440 |
| Rmerge | 0.178 | 0.544 |
| Rmeas | 0.216 | 0.670 |
| Rpim | 0.120 | 0.384 |
| Total number of observations | 410595 | 15789 |
| Number of reflections | 135777 | 6315 |
| <I/σ(I)> | 6.4 | 1.5 |
| Completeness [%] | 97.6 | |
| Redundancy | 3 | 2.5 |
| CC(1/2) | 0.966 | 0.792 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM |
