9AVW

Structure of TAB2 NZF domain bound to K6 / Lys6-linked diubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96861
Spacegroup name	P 65 2 2
Unit cell lengths	89.663, 89.663, 86.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.831 - 1.747
R-factor	0.1948
Rwork	0.194
R-free	0.22150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.880
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.831	1.839
High resolution limit [Å]	1.747	1.747
Rpim	0.089	0.248
Number of reflections	21100	2714
<I/σ(I)>	7.2	2.3
Completeness [%]	99.2	96.8
Redundancy	18.6	16.6
CC(1/2)	0.990	0.523

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	2.2 M ammonium sulphate, 20% glycerol