9AVT

Structure of TAB2 NZF domain bound to K6 / Lys6-linked diubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96861
Spacegroup name	P 65 2 2
Unit cell lengths	90.323, 90.323, 86.335
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.020 - 1.500
R-factor	0.1835
Rwork	0.182
R-free	0.20330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.351
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.160	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.041	1.028
Rmeas	0.045	1.123
Rpim	0.018	0.445
Total number of observations	217524	10260
Number of reflections	33838	1646
<I/σ(I)>	20	1.8
Completeness [%]	100.0
Redundancy	6.4	6.2
CC(1/2)	0.999	0.636

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.2 M ammonium sulphate