9AVK
Structure of long Rib domain from Limosilactobacillus reuteri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2021-07-16 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 36.161, 36.161, 126.332 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.800 - 1.460 |
| R-factor | 0.15536 |
| Rwork | 0.154 |
| R-free | 0.18257 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.648 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.800 | 1.560 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.059 | 0.343 |
| Number of reflections | 14807 | 2036 |
| <I/σ(I)> | 20.2 | |
| Completeness [%] | 95.4 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 2.4 M Na Malonate pH 7, 0.2 M NaSCN |
