9AVF

CCHFV GP38-GnH-DS heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-17
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	63.809, 87.010, 128.759
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.750 - 2.500
R-factor	0.1987
Rwork	0.197
R-free	0.23740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.836
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5127)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.750	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	25418	2446
<I/σ(I)>	10.4	1.8
Completeness [%]	99.6
Redundancy	6.5
CC(1/2)	0.992	0.546

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295.15	8% (v/v) TacsimateTM pH 8.0, 20% (v/v) PEG 3,350