9AVE

Mitochondrial fission 1 protein Fis1 T34E mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 41 21 2
Unit cell lengths	43.250, 43.250, 119.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.070 - 2.370
R-factor	0.2422
Rwork	0.239
R-free	0.29070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.085
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.100	35.100	2.430
High resolution limit [Å]	2.370	10.600	2.370
Rmerge	0.060	0.030	2.036
Rmeas	0.063	0.032	2.136
Number of reflections	5063	75	357
<I/σ(I)>	18.74
Completeness [%]	100.0
Redundancy	10.6
CC(1/2)	1.000	1.000	0.654

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	0.2M Ammonium Acetate, 0.1M HEPES (pH 7.5), 25% PEG 3350