9AVC

Fis1 Structure Y38E mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	43.470, 43.070, 61.240
Unit cell angles	90.00, 99.34, 90.00

Refinement procedure

Resolution	38.010 - 2.090
R-factor	0.1926
Rwork	0.190
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.896
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.100	38.100	2.140
High resolution limit [Å]	2.090	9.350	2.090
Rmerge	0.048	0.022	1.443
Rmeas	0.052	0.024	1.565
Number of reflections	13234	169	967
<I/σ(I)>	18.77
Completeness [%]	98.2
Redundancy	6.7
CC(1/2)	0.999	0.999	0.605

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.1M Sodium phosphate dibasic dihydrate, 20% PEG 3350