9AVB

Fis1 Wild type

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98726
Spacegroup name	P 65
Unit cell lengths	58.330, 58.330, 156.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.910 - 1.950
R-factor	0.1869
Rwork	0.185
R-free	0.23060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.963
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.910	30.910	2.000
High resolution limit [Å]	1.950	8.720	1.950
Rmerge	0.089	0.035	2.448
Rmeas	0.092	0.037	2.543
Number of reflections	21571	246	1587
<I/σ(I)>	18.55
Completeness [%]	98.6
Redundancy	13.8
CC(1/2)	0.999	0.999	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	25% PEG 5KMME, 0.15M NaCl, 0.033M HEPES(pH 7.5), 0.033M ADA (pH 6.5), 0.033M Tris.HCl (pH 8.0)