9AUR

Crystal structure of loop-closed A21 2'-OMe dumbbell RNA bridged by glycine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-04
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979180
Spacegroup name	P 1 21 1
Unit cell lengths	39.466, 83.126, 73.339
Unit cell angles	90.00, 90.25, 90.00

Refinement procedure

Resolution	54.990 - 1.570
R-factor	0.1868
Rwork	0.186
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.993
Data reduction software	MOSFLM (1.20.1_4487)
Data scaling software	SCALEPACK
Phasing software	PHASER (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.990	1.626
High resolution limit [Å]	1.570	1.570
Number of reflections	65261	6414
<I/σ(I)>	9.31
Completeness [%]	96.4
Redundancy	3.4
CC(1/2)	0.903	0.488

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.8	277.15	0.07 M Citric Acid; 0.03 M BIS-TRIS Propane; 16% w/v Polyethylene glycol 3,350