9AUD
Immune receptor complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | H 3 |
| Unit cell lengths | 196.344, 196.344, 77.104 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.370 - 2.900 |
| R-factor | 0.1847 |
| Rwork | 0.183 |
| R-free | 0.21650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.453 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.370 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.081 | 0.323 |
| Rmeas | 0.091 | 0.366 |
| Rpim | 0.042 | 0.169 |
| Number of reflections | 24545 | 3973 |
| <I/σ(I)> | 11 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.8 | 4.7 |
| CC(1/2) | 0.976 | 0.775 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 0.2 M Sodium Acetate, 0.1 M BIS-TRIS prop pH 8.5, 20 %w/v PEG 3350 |
