9ARR

Crystal structure of AF9 YEATS domain in complex with dicrotonylated at K1007 and K1014 MOZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2023-06-09
Detector	RDI CMOS_8M
Wavelength(s)	1.0722
Spacegroup name	C 1 2 1
Unit cell lengths	143.364, 49.021, 78.133
Unit cell angles	90.00, 113.64, 90.00

Refinement procedure

Resolution	45.970 - 2.100
R-factor	0.20529
Rwork	0.203
R-free	0.24748
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.649
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.970	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.056	0.632
Rmeas	0.066	0.754
Rpim	0.034	0.407
Total number of observations		7865
Number of reflections	27648	2370
<I/σ(I)>	8.7	1.7
Completeness [%]	94.2
Redundancy	3.6	3.3
CC(1/2)	0.999	0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		291	0.2 M Lithium nitrate pH 7.1, 20% w/v PEG 3350