9ABP
A PRO TO GLY MUTATION IN THE HINGE OF THE ARABINOSE-BINDING PROTEIN ENHANCES BINDING AND ALTERS SPECIFICITY: SUGAR-BINDING AND CRYSTALLOGRAPHIC STUDIES
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.460, 71.820, 77.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.970 |
| R-factor | 0.18 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.005 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.970 * |
| Rmerge | 0.037 * |
| Total number of observations | 35161 * |
| Number of reflections | 17864 * |
| Completeness [%] | 79.0 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | PEG | 10 (%) | |
| 3 | 1 | drop | citrate | 10 (mM) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | sodium azide | 0.02 (%) | |
| 6 | 1 | drop | D-galactose | 10 (mM) | |
| 7 | 1 | reservoir | PEG | 20 (%) |
