8H0S

Crystal structure of MnmM from B. subtilis complexed with Gln-TTG anti-codon stem loop and SAM (2.90 A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	43.180, 60.755, 94.059
Unit cell angles	90.97, 93.10, 101.55

Refinement procedure

Resolution	93.880 - 2.900
R-factor	0.24281
Rwork	0.241
R-free	0.28520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pon
RMSD bond length	0.005
RMSD bond angle	1.263
Data reduction software	XDS (Jan 10, 2022)
Data scaling software	STARANISO
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.880	3.080
High resolution limit [Å]	2.900	2.900
Rmerge	0.165	0.995
Rmeas	0.195	1.169
Rpim	0.102	0.607
Number of reflections	18875	3264
<I/σ(I)>	6.6	1.2
Completeness [%]	91.2	97.5
Redundancy	3.5	3.6
CC(1/2)	0.992	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dehydrate pH5.6, 30% w/v Polyethylene glycol 4,000, 3% w/v Trimethylamine N-oxide dihydrate