8H0S
Crystal structure of MnmM from B. subtilis complexed with Gln-TTG anti-codon stem loop and SAM (2.90 A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-29 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 43.180, 60.755, 94.059 |
Unit cell angles | 90.97, 93.10, 101.55 |
Refinement procedure
Resolution | 93.880 - 2.900 |
R-factor | 0.24281 |
Rwork | 0.241 |
R-free | 0.28520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pon |
RMSD bond length | 0.005 |
RMSD bond angle | 1.263 |
Data reduction software | XDS (Jan 10, 2022) |
Data scaling software | STARANISO |
Phasing software | MOLREP (11.9.02) |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 93.880 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.165 | 0.995 |
Rmeas | 0.195 | 1.169 |
Rpim | 0.102 | 0.607 |
Number of reflections | 18875 | 3264 |
<I/σ(I)> | 6.6 | 1.2 |
Completeness [%] | 91.2 | 97.5 |
Redundancy | 3.5 | 3.6 |
CC(1/2) | 0.992 | 0.768 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dehydrate pH5.6, 30% w/v Polyethylene glycol 4,000, 3% w/v Trimethylamine N-oxide dihydrate |