8BNA
BINDING OF HOECHST 33258 TO THE MINOR GROOVE OF B-DNA
Experimental procedure
| Detector technology | DIFFRACTOMETER |
| Detector | SYNTEX P1 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 25.040, 40.330, 65.850 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 8.000 - 2.200 |
| R因子 | 0.14 * |
| Refinement software | JACK-LEVITT |
Quality characteristics
| Overall | |
| 分解能 [Å] (高) | 2.200 |
| 独立反射数 | 3487 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION | 7 * | 277 | VAPOR DIFFUSION, temperature 277.00K |
結晶化試薬
| ID | 結晶ID | 濃度 | 試薬名 | 濃度 (単位) | 詳細 |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MG ACETATE | ||
| 4 | 1 | 1 | SPERMINE ACETATE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | DNA | 0.21 (mM) | |
| 2 | 1 | drop | Hoechst 33258 | 0.21 (mM) | |
| 3 | 1 | drop | 5.38 (mM) | ||
| 4 | 1 | drop | spermine acetate | 0.21 (mM) | |
| 5 | 1 | drop | MPD | 20 (%(w/v)) | |
| 6 | 1 | reservoir | 35 (%) |
