8ZYK

Crystal structure of hemagglutinin from HN/4-10 H3N8 influenza virus S228 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03961
Spacegroup name	P 21 21 21
Unit cell lengths	136.829, 167.321, 206.841
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.530 - 3.010
R-factor	0.2399
Rwork	0.238
R-free	0.27790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.481
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge		1.251
Number of reflections	93878	93878
<I/σ(I)>	8.3
Completeness [%]	96.2
Redundancy	10
CC(1/2)	0.748	0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	28% v/v 2-Propanol, 0.1 M BIS-TRIS pH 6.5, 3% v/v Polyethylene glycol 200