8ZYF

Crystal structure of ZW2G10 Fab in complex with omicron RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2022-08-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	44.664, 103.529, 299.352
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.530 - 2.600
R-factor	0.2348
Rwork	0.232
R-free	0.28470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.070
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((1.17_3644: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.530	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	43011	7857
<I/σ(I)>	16
Completeness [%]	87.4
Redundancy	5.7
CC(1/2)	0.984	0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2M Ammonium acetate, 0.1M MES pH 6.5, 15% w/v Polyethylene glycol 3,350