8ZYB

a-KG dependent oxygenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-14
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	368.944, 78.254, 81.912
Unit cell angles	90.00, 98.02, 90.00

Refinement procedure

Resolution	30.570 - 2.580
R-factor	0.2042
Rwork	0.203
R-free	0.25730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	7.259
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.570	2.670
High resolution limit [Å]	2.580	2.580
Rpim	0.077	0.077
Number of reflections	71023	5961
<I/σ(I)>	12.26
Completeness [%]	97.5
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.15	0.2M magnesium formate, 22.58% w/v polyethylene glycol 3350