8ZXY

sweet protein MNEI-Mut 6-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.94138
Spacegroup name	P 21 21 21
Unit cell lengths	51.426, 58.697, 66.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.040 - 1.430
R-factor	0.1558
Rwork	0.154
R-free	0.19510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iv7
RMSD bond length	0.015
RMSD bond angle	1.365
Data reduction software	HKL-3000
Phasing software	PHENIX (1.19_4092)
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.040	1.480
High resolution limit [Å]	1.430	1.430
Number of reflections	37689	37657
<I/σ(I)>	51.9
Completeness [%]	99.0
Redundancy	9.2
CC(1/2)	0.980	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	PEG 6000 30% BIS-TRIS Propone