8ZXW
Crystal structure of the anti-phosphorylated peptide C7 Fab antibody with peptide bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.351, 67.841, 71.341 |
| Unit cell angles | 112.54, 104.99, 98.31 |
Refinement procedure
| Resolution | 48.040 - 1.330 |
| Rwork | 0.137 |
| R-free | 0.16530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.532 |
| Data reduction software | XDS (20230630) |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.040 | 1.350 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.043 | 0.463 |
| Rpim | 0.027 | 0.296 |
| Number of reflections | 188006 | 6654 |
| <I/σ(I)> | 15.4 | 2.2 |
| Completeness [%] | 93.5 | 66.6 |
| Redundancy | 3.5 | 3.3 |
| CC(1/2) | 0.998 | 0.781 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M Sodium chloride 19% Polyethylene glycol 3350 |
