8ZXV

sweet protein MNEI-Mut 6-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.94138
Spacegroup name	P 21 21 21
Unit cell lengths	51.318, 58.560, 66.139
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.800 - 1.430
R-factor	0.1729
Rwork	0.171
R-free	0.20280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iv7
RMSD bond length	0.015
RMSD bond angle	1.799
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.800	1.450
High resolution limit [Å]	1.430	1.430
Number of reflections	37636	37583
<I/σ(I)>	47.4
Completeness [%]	99.0
Redundancy	11.4
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		286	PEG 6000 30% BIS-TRIS Propone