8ZXO
Structure of the red fluorescent protein mScarlet-H at pH8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.715, 45.351, 58.658 |
| Unit cell angles | 90.00, 102.10, 90.00 |
Refinement procedure
| Resolution | 41.420 - 1.816 |
| R-factor | 0.188 |
| Rwork | 0.184 |
| R-free | 0.22610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.13-2998) |
| Refinement software | PHENIX (1.13-2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.360 | 1.910 |
| High resolution limit [Å] | 1.816 | 1.820 |
| Rmerge | 0.167 | |
| Rmeas | 0.206 | |
| Rpim | 0.120 | |
| Number of reflections | 19465 | 2846 |
| <I/σ(I)> | 4.2 | |
| Completeness [%] | 98.2 | 99.5 |
| Redundancy | 2.7 | 2.7 |
| CC(1/2) | 0.975 | 0.511 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.0M Ammonium sulfate,0.1M tris8.5 |
